mouse anti mouse nk1 1 monoclonal antibody  (Bio X Cell)

Journal: Oncoimmunology

Article Title: Sex determines the natural killer cell-mediated immunity against pancreatic cancer

doi: 10.1080/2162402X.2026.2645298

Figure Lengend Snippet: Panc02 allograft model shows reduced tumor growth but enhanced NK cell infiltration in female mice. (A) Schematic illustration of the experimental design. (B) Growth curves of male and female tumors (1) and representative images of excised tumors (2). Tumor volume at termination (3). Statistical significance was determined by Mann–Whitney U test, n = 5/sex. (C) Representative images for immunohistochemical staining for NK1.1; males vs. females; arrows indicate infiltrating NK cells (1). Scatter dot plot showing NK cell counts per area; statistical significance was determined by Mann–Whitney U test (2). Heatmap of RNA-seq expression for tumor and NK cell markers (3), n = 5/sex. (D) Correlation between NK cell infiltration and tumor size, simple linear regression and one-tailed Pearson correlation, n = 5/sex.

Article Snippet: The suspension was filtered, and after erythrocyte-lysis, stained with NK1.1 and CD3 antibodies (Miltenyi Biotec) as well as with DAPI live/dead staining and sorted by fluorescence-activated cell sorting (FACS) (BD FACS Aria II, BD Biosciences) for DAPI−NK1.1 + CD3− cells.

Techniques: MANN-WHITNEY, Immunohistochemical staining, Staining, RNA Sequencing, Expressing, One-tailed Test

Journal: bioRxiv

Article Title: In vivo screening reveals new regulators of Natural Killer cell development and functional response to acute cytomegalovirus infection

doi: 10.64898/2026.03.19.709783

Figure Lengend Snippet: (a) MCMV titers in the spleen 4 days after infection with tissue culture-derived MCMV +/− anti-NK1.1 administration. Data are merged from two experiments and represent 4 in total. (b & c) Representative FACS plots (b) and quantification (c) of degranulating NK cells from spleens of WT and Sytl3 −/− mice 4 days after MCMV infection. Data represent > 3 experiments. (d) Externalization of CD107a was assessed by flow cytometry following stimulation of NK cells from WT and Sytl3 −/− mice +/− PMA-Ionomycin, cytokines or P815 cells pre-incubated +/− agonist antibodies to activating NK cell receptors. Cells were pooled within groups where lymphocyte numbers were insufficient. Individual mice (except 1 point which represents 2 pooled mouse samples), median and interquartile range are shown. Data from 1 of 3 experiments are shown. Significance was assessed using paired analysis of WT and Sytl3 −/− NK cells in each treatment condition using a Mann Whitney U test. *p < 0.05, ** p = 0.005. (e) NK cells derived from WT and Sytl3 −/− mice were stimulated with BaF/3 control and BaF/3-m157 cells across several E:T ratios. Annexin V binding to target cells was assessed. Mean +/− SD is shown from 3 technical replicates/data point. Paired statistical analyses of WT vs Sytl3 −/− was performed using Student’s t-Test at each E:T ratio for either m157 + or m157 − cell lines. *p < 0.05. (f) Representative microscopy images of F-actin (red), brightfield and interference reflection microscopy [IRM] (shown in grayscale) in naïve WT and Sytl3 −/− NK cells stimulated for 10 minutes on surfaces coated with anti-NK1.1 mAb. Scale bars, 10 µm. (g) The proportion of cells that have formed an actin ring. Data represents 2 experiments. (h) Degranulation of human primary blood expanded NK cells in response to K562 or PMA/Ionomycin stimulation and (i) specific killing of K562 targets after 3-hour incubation with NK cells. STC is a safe cutting control and SYLT3_g1 and SYLT3_g4 are two independent sgRNA to generate SYLT3 KO. (h-i) Each dot represents an individual human donor, p values from 2-way ANOVA with correction for multiple testing with a post-hoc Dunnett test. Representative image from two independent experiments with unique donors.

Article Snippet: Some mice were treated with 200μg anti-NK1.1 (clone PK136, BioXCell) or PBS control on days −2, 0 and +2 post infection Age- and sex-matched mice (males and females) aged 6-12 weeks were used.

Techniques: Infection, Derivative Assay, Flow Cytometry, Incubation, MANN-WHITNEY, Control, Binding Assay, Microscopy